Fermentative preparation of S35-siomycin, a sulfur-containing peptide antibiotic.

rpm). An inoculum of 5-8 % of the rusulting seed broth was then used to inoculate the fermentation flasks. The composition of the fermentation mediumwas as follows : sucrose 7 %, soybean meal 2.5 %, ammonium sulfate 0.3 %, pH 7.0, 25 ml of medium being used per 200ml flask. The fermentation was carried out on a rotary shaker at 200 rpm for about 10 days at 30°C. Feeding of S35-ammonium sulfate solution (see above)